Primer3 Output

No mispriming library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq 
LEFT PRIMER         89   20   60.95   55.00  5.00  2.00 TCTGTCACACCGACTTGCAC
RIGHT PRIMER       310   20   59.05   55.00  4.00  2.00 AGTCAGCGTGAGGACAGTTG
SEQUENCE SIZE: 325
INCLUDED REGION SIZE: 325

PRODUCT SIZE: 222, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 3.00
TARGETS (start, len)*: 118,171

    1 GAATCCCACGGTAAATTGGAACACAAGGATATTCCAGTTCCAAAGCCAAAGGCCAACGAA
                                                                  

   61 TTGTTGATCAACGTTAAGTACTCTGGTGTCTGTCACACCGACTTGCACGCTTGGCACGGT
                                  >>>>>>>>>>>>>>>>>>>>         ***

  121 GACTGGCCATTGCCAGTTAAGCTACCATTAGTCGGTGGTCACGAAGGTGCCGGTGTCGTT
      ************************************************************

  181 GTCGGCATGGGTGAAAACGTTAAGGGCTGGAAGATCGGTGACTACGCCGGTATCAAATGG
      ************************************************************

  241 TTGAACGGTTCTTGTATGGCCTGTGAATACTGTGAATTGGGTAACGAATCCAACTGTCCT
      ************************************************  <<<<<<<<<<

  301 CACGCTGACTTGTCTGGTTACACCC
      <<<<<<<<<<               

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq 

 1 LEFT PRIMER         87   20   60.95   55.00  5.00  2.00 TGTCTGTCACACCGACTTGC
   RIGHT PRIMER       310   20   59.05   55.00  4.00  2.00 AGTCAGCGTGAGGACAGTTG
   PRODUCT SIZE: 224, PAIR ANY COMPL: 8.00, PAIR 3' COMPL: 3.00

 2 LEFT PRIMER         84   20   60.21   55.00  5.00  3.00 TGGTGTCTGTCACACCGACT
   RIGHT PRIMER       307   19   61.04   57.89  3.00  0.00 CAGCGTGAGGACAGTTGGA
   PRODUCT SIZE: 224, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00

 3 LEFT PRIMER         89   20   60.95   55.00  5.00  2.00 TCTGTCACACCGACTTGCAC
   RIGHT PRIMER       311   21   60.49   52.38  4.00  2.00 AAGTCAGCGTGAGGACAGTTG
   PRODUCT SIZE: 223, PAIR ANY COMPL: 8.00, PAIR 3' COMPL: 3.00

 4 LEFT PRIMER         89   20   60.95   55.00  5.00  2.00 TCTGTCACACCGACTTGCAC
   RIGHT PRIMER       309   20   61.90   60.00  4.00  0.00 GTCAGCGTGAGGACAGTTGG
   PRODUCT SIZE: 221, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00

Statistics
         con   too    in    in          no    tm    tm  high  high        high      
         sid  many   tar  excl   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   GC% clamp   low  high compl compl     X  stab    ok
Left     836     0     0     0     0     0   278   300     0     9     0    14   235
Right    143     0     0     0     0     0    32    67     0     3     0     3    38
Pair Stats:
considered 1010, unacceptable product size 891, high any compl 66, high end compl 36, ok 17
primer3 release 1.0

(primer3_www_results.cgi v 0.4)